| 质粒名称: | pAM-PAT-ProGL2 GW |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 80689 |
| 存储实验室: | Nico Dissmeyer, Arp Schnittger |
|
载体骨架基本信息 |
|
| 载体名称: | Binary vector pAMPAT-MCS |
| 完整质粒大小: | 8454 bp |
| 载体类型: | 植物表达 |
| 筛选标记: | Basta |
| 载体抗性: | Chloramphenicol and Ampicillin |
| 生长温度: | 37 ℃ |
| 宿主菌: | ccdB Survival |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 启动子: | ProGL2 |
| 克隆方法: | Gateway Cloning |
| 5’测序引物 | gaccggcaacaggattcaatcttaag |
| 3’测序引物 | GAATTAGAAATTTTATTGATAGAAG |
|
文献引用方法 |
|
| 材料和方法部分: |
pAM-PAT-ProGL2 GW was a gift from Nico Dissmeyer & Arp Schnittger (Addgene plasmid # 80689 ; http://n2t.net/addgene:80689 ; RRID:Addgene_80689)
|
| 参考文献部分: | Novel functions of plant cyclin-dependent kinase inhibitors, ICK1/KRP1, can act non-cell-autonomously and inhibit entry into mitosis. Weinl C, Marquardt S, Kuijt SJ, Nowack MK, Jakoby MJ, Hulskamp M, Schnittger A. Plant Cell. 2005 Jun;17(6):1704-22. Epub 2005 Mar 4. 10.1105/tpc.104.030486 PubMed 15749764 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAM-PAT-ProGL2 GW 8454 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION binary DEST vector for plant expression.
ACCESSION .
VERSION .
KEYWORDS pAM-PAT-ProGL2 GW
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8454)
AUTHORS Weinl C, Marquardt S, Kuijt SJ, Nowack MK, Jakoby MJ, Hulskamp M,
Schnittger A
TITLE Novel functions of plant cyclin-dependent kinase inhibitors,
ICK1/KRP1, can act non-cell-autonomously and inhibit entry into
mitosis.
JOURNAL Plant Cell. 2005 Jun;17(6):1704-22. Epub 2005 Mar 4.
PUBMED 15749764
REFERENCE 2 (bases 1 to 8454)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8454
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 2..26
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
terminator 120..372
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(392..943)
/codon_start=1
/gene="Streptomyces hygroscopicus bar"
/product="phosphinothricin acetyltransferase"
/label=BlpR
/note="confers resistance to bialophos or phosphinothricin"
/translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
WQLDFSLPVPPRPVLPVTEI"
promoter complement(985..1164)
/label=NOS promoter
/note="nopaline synthase promoter"
protein_bind 3439..3563
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 3588..3618
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 3672..4331
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(3738..3757)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 4673..4978
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 4857..4876
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(5019..5143)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
misc_feature 5514..5538
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin 5650..6359
/label=oriV
/note="incP origin of replication"
promoter 6433..6524
/gene="bla"
/label=AmpR promoter
CDS 6525..7385
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6743..6762)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7556..8144
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8045..8064
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc
61 ggacgttttt aatgatcgaa tactaacgtc tctaccagat atcagcttgc atgccggtcg
121 atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt
181 gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat
241 aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta
301 tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat
361 gtttgaacga tctgcttgac tctaggggtc atcagatttc ggtgacgggc aggaccggac
421 ggggcggcac cggcaggctg aagtccagct gccagaaacc cacgtcatgc cagttcccgt
481 gcttgaagcc ggccgcccgc agcatgccgc ggggggcata tccgagcgcc tcgtgcatgc
541 gcacgctcgg gtcgttgggc agcccgatga cagcgaccac gctcttgaag ccctgtgcct
601 ccagggactt cagcaggtgg gtgtagagcg tggagcccag tcccgtccgc tggtggcggg
661 gggagacgta cacggtcgac tcggccgtcc agtcgtaggc gttgcgtgcc ttccagggac
721 ccgcgtaggc gatgccggcg acctcgccgt ccacctcggc gacgagccag ggatagcgct
781 cccgcagacg gacgaggtcg tccgtccact cctgcggttc ctgcggctcg gtacggaagt
841 tgaccgtgct tgtctcgatg tagtggttga cgatggtgca gaccgccggc atgtccgcct
901 cggtggcacg gcggatgtcg gccgggcgtc gttctgggct catggtagat cccctcgatc
961 gagttgagag tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt
1021 ggagcatttt tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg
1081 cgcaataatg gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctca
1141 gtggctcctt caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc
1201 atcggcgggg gtcataacgt gactccctta attctccgct catgatcaga ttgtcgtttc
1261 ccgccttcgg tttgggcgcg ccaagctttt gaattgtaga taaatcatct gctacagtta
1321 taccattata tatcttatta aagacctaag tttccttcac tatacgtctt cgtccattta
1381 cgtacgtatt atacggacgg tttaagctac tatatctata ttgttaacaa tgtaactgtt
1441 gagatatatc ttgcaataat atgtcatggt gtatgcatac gataatatga atcaatgttt
1501 gaaatcttga cgtgcccgtg atacaataag atgatcaaaa tttcaaattt tgtcaaatat
1561 taaaacaaca tacacataca catgtgtcca ggtggcatta taaaatgtat atatggtgga
1621 tatagagaga gagggagatg cgtatagtga ataggaaagt aagtaataaa gagagggtgg
1681 aggaattgga aaggggttgg aggcaaaccc ataaagagca ttcatttcct tttaaggtcg
1741 ctgaaattaa tgagtaacga tcggtcaatg cctctcgctg acctttttct ttttttacaa
1801 caacaaataa aaataaaata aatttcgacg tctctttccg ctgctgaatt acatttgttg
1861 aattaatttt ctctgcttac gtacgtcttc taaactttct ctatccgaat tcttttttaa
1921 ctttctaact tatattcaac aactcttctt tcctgccttt accgttagtc taattgtttt
1981 cctaatactg ctacgtacat acccctacta tactagtcag tgtattagat tcgattggga
2041 ttaatccagg aatatagata tcccattagt ttttataaaa atattggaag aggacaagtc
2101 tcaagcaatt tagggttcca tgtagcgctg caatatactg ttagtaactc tctcttaccc
2161 atatattgta tatgctaatt cttatcaaat atatatatat gcttctccca gagtcccagt
2221 ttcctataat cctgacgcaa ttatactaat agagccaagt ttacataata aagtatatat
2281 gattaataga tagggtttct tattaagcca tatcttaaat taagatgtga tgatagcgtt
2341 ttgtataagt taccaattgt ttgaaagaag agatcatcac aataataaat cataagtagt
2401 agtatatagt aataaataaa tacacaagtc ataataagag taatgagagg ataattaagg
2461 agggaagaag aaagcagaaa atgcggttgg agaattaggt gctaaaagtt agttgagtcc
2521 atctcagtat ctaacggtca actctctctc tctctagaga aaacaattaa gaaatctgac
2581 atacacatat gtctctctct ctctctctct ctagtctata cacacaattc aattaaagaa
2641 gagacagaga agttcgtctt ttttgttttt atacccttaa atcaatcatg caattgtaac
2701 ccttccttct tattctcatt ccttcccccc ctgtctacag taatctatag caacgccatt
2761 atgtactact tttaacggat aatttgctca tgtttcaata tggcttcact gtatatatgt
2821 tcaagttctt ctcaatccct ttatatcatt ccaacataat tcatattaaa gttagtagct
2881 gaaattggaa ggctgatata ttttccataa ttcaaatttg aattttgctc atcatatata
2941 tatgtatata ttaaaaatcg aatattaaga agaaaaatga agtcgatcga tggctgccaa
3001 tgctgtagct ggccatgttt taaactactc aattgtcgga ttgaagtata gccaaaatat
3061 ataaaaccgt aaaaggacta aatataataa tataataggt attaattaat taaaactaat
3121 taattataaa agaagcacct aaaagtcaag agcagtagag aaatggaaga aatatctgaa
3181 aaacgaccgc ttatatatat atgtatcatt ggaattgaag aggctatata tatatatata
3241 tatatatata tatatatatc gatcttagct tatatattaa ttgaaagtac attttggtgt
3301 ataagtaatt aaagaagaaa gaaaaaaaga gagataatat ataaggaaga aggagtgcga
3361 ggagaagagg gaagagatca taattaagca aagaagctag aggatccttg ctcgagaaag
3421 aggatccacc tgaggatcac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat
3481 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
3541 acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg
3601 cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta
3661 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
3721 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
3781 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
3841 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
3901 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
3961 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
4021 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
4081 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
4141 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
4201 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg
4261 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
4321 gcggggcgta aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc
4381 gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa
4441 gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct
4501 caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc
4561 cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc
4621 cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg aaatgcagtt
4681 taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga
4741 tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc
4801 agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat
4861 gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct
4921 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat
4981 gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg
5041 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
5101 tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggtgatcctc cgttccatgg
5161 gctagaagct tgttgagaat tcatcgcccg ggactgggtt aacttccgat cgattctaga
5221 ctagttctag agtccgcaaa aatcaccagt ctctctctac aaatctatct ctctctattt
5281 ttctccagaa taatgtgtga gtagttccca gataagggaa ttagggttct tatagggttt
5341 cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc
5401 tatcaataaa atttctaatt cctaaaacca aaatccagtg accgggcggc cgccaccgcg
5461 gtggaggggg atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg
5521 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa
5581 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg tacatcaccg acgagcaagg
5641 caagaccgag cgcctttccg acgctcaccg ggctggttgc cctcgccgct gggctggcgg
5701 ccgtctatgg ccctgcaaac gcgccagaaa cgccgtcgaa gccgtgtgcg agacaccggc
5761 cgccggcgtt gtggatacct cgcggaaaac ttggccctca ctgacagatg aggggcggac
5821 gttgacactt gaggggccga ctcacccggc gcggcgttga cagatgaggg gcaggctcga
5881 tttcggccgg cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac
5941 gcgagtttcc cacagatgat gtggacaagc ctggggataa gtgccctgcg gtattgacac
6001 ttgaggggcg cgactactga cagatgaggg gcgcgatcct tgacacttga ggggcagagt
6061 gctgacagat gaggggcgca cctattgaca tttgaggggc tgtccacagg cagaaaatcc
6121 agcatttgca agggtttccg cccgtttttc ggccaccgct aacctgtctt ttaacctgct
6181 tttaaaccaa tatttataaa ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg
6241 cgcacgccga aggggggtgc ccccccttct cgaaccctcc cggcccgcta acgcgggcct
6301 cccatccccc caggggctgc gcccctcggc cgcgaacggc ctcaccccaa aaatggcagc
6361 gctggcagtc cttgccattg ccgggatcgg ggcagtaacg ggatgggcga tcagcccgac
6421 aagctacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
6481 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
6541 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
6601 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
6661 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
6721 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
6781 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
6841 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
6901 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
6961 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
7021 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
7081 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
7141 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
7201 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
7261 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
7321 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
7381 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
7441 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
7501 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
7561 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
7621 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
7681 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
7741 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
7801 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
7861 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
7921 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
7981 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
8041 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
8101 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
8161 cctttttacg gttcctggcc ttttgctggc cttttgctca catggactct agctagagga
8221 tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc
8281 cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca
8341 aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg
8401 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctg
//
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